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How to Calculate Peptide Dosage

A comprehensive guide to calculating peptide dosage, injection volumes, and reconstitution ratios using standard unit conversions and mathematical formulas.

Introduction

Calculating peptide dosage accurately is essential for safe and effective peptide use. This guide will walk you through the process of determining injection volumes, understanding concentrations, and converting between different units (mg, mcg, ml, and IU).

Whether you're working with research peptides or need to calculate dosages for specific protocols, understanding these calculations will help you prepare accurate injections.

Understanding the Units

Before calculating peptide dosages, it's important to understand the units involved:

  • Milligrams (mg): Used to measure the total amount of peptide in a vial (e.g., 5 mg, 10 mg)
  • Micrograms (mcg): Used to measure individual doses (e.g., 250 mcg, 500 mcg). 1 mg = 1000 mcg
  • Milliliters (ml): Used to measure liquid volume, including bacteriostatic water and injection volumes
  • International Units (IU): A standardized measurement, often calculated as ml × 100

Step-by-Step Calculation Process

Step 1: Determine Your Peptide Amount

Check your peptide vial label for the total amount in milligrams (mg). Common vial sizes include 2 mg, 5 mg, and 10 mg.

Step 2: Choose Your Reconstitution Volume

Decide how much bacteriostatic water to add. Common volumes range from 1 ml to 3 ml per 5 mg vial. More water creates a lower concentration but larger injection volumes, while less water creates a higher concentration with smaller volumes.

Step 3: Calculate Concentration

Use this formula to determine the concentration:

Concentration (mg/ml) = Peptide amount (mg) ÷ Water volume (ml)

Step 4: Determine Your Desired Dose

Based on your protocol or research, determine your desired dose per injection in micrograms (mcg). Common doses range from 100-500 mcg depending on the peptide.

Step 5: Calculate Injection Volume

Convert your dose to mg and calculate the injection volume:

Dose in mg = Desired dose (mcg) ÷ 1000

Injection volume (ml) = Dose (mg) ÷ Concentration (mg/ml)

Complete Example Calculation

Scenario: You have a 5 mg peptide vial and want to inject 250 mcg per dose.

Given Values:

  • Peptide amount: 5 mg
  • Water to add: 2 ml
  • Desired dose: 250 mcg

Calculation Steps:

  1. Concentration = 5 mg ÷ 2 ml = 2.5 mg/ml
  2. Dose in mg = 250 mcg ÷ 1000 = 0.25 mg
  3. Injection volume = 0.25 mg ÷ 2.5 mg/ml = 0.1 ml
  4. IU = 0.1 ml × 100 = 10 IU
  5. Total injections = 5 mg ÷ 0.25 mg = 20 injections

Result:

Draw 0.1 ml (10 IU) for each injection. This vial will provide 20 total injections at 250 mcg per dose.

Common Dosage Ranges

While dosages vary by peptide type, here are common ranges:

Low Dose Range

100-200 mcg per injection

Medium Dose Range

250-400 mcg per injection

High Dose Range

500-1000 mcg per injection

Note

Always consult research literature or healthcare professionals for specific peptide dosages

Important Safety Considerations

  • Always use sterile, pharmaceutical-grade bacteriostatic water for reconstitution
  • Follow proper aseptic techniques when handling peptides and reconstituting solutions
  • Store reconstituted peptides according to manufacturer guidelines (typically refrigerated)
  • Double-check all calculations before administration
  • Use appropriate syringe sizes for accurate measurement of small volumes
  • Consult with healthcare professionals for proper dosing guidance
  • These calculations are for educational and research purposes only

Using Our Calculators

Our online calculators simplify this process by automatically performing all calculations:

Simply enter your values and the calculators will provide instant results, eliminating the need for manual calculations.